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Artisanal wooden jigsaw puzzles made in the USA.

Event website: http://www.stjameschamber.net/sip-n-savor

Savana Live Live at Blumenhof Winery
Saturday, May 6, 2017 - 2:00pm to 5:00pm
Blumenhof Winery

Savana (Rock Band) will perform at Blumenhof Winery in Dutzow, MO on Saturday, May 6 from 2-5pm.

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Saturday, May 6, 2017 - 2:00pm to 5:00pm
Tipple Hill Winery

Pre-Sales 1:30 2:00 to 5:00

Quarter Auction Fundraiser -For Cystic Fibrosis Foundation supporting our Bridget. She is recovering froma double lung transplant.

What is a Quarter Auction, you ask? You purchase a bidding paddle for $5 anduse it to bid on donated items from home based businesses and other itemssuch as gift cards, wine baskets, handmade craft items, and so much more.When an item comes up for bid that you are interested in, you simple holdyour bidding paddle up, deposit a quarter or two (depending on the value ofthe item) into the bucket. A number is drawn and if it is your paddle numberyou win!We will have several home based businesses also selling theiritems. Vendors present will be:Pampered Chef, LulaRoe, Lipsense,OrigamiOwl, Perfectly Posh, Scentsy, Tupperware, and more.

Event URL: https://www.tipplehillwinery.com/events

Third Annual Kansas City English
Saturday, May 6, 2017 - 4:00pm to 6:00pm
Riverwood Winery

On this Saturday, a day of Wine Races! Join us for wine tasting, KentuckyDerby games, bake sale, bulldog kissing booth and more (bring cash for thegames and bake sale)!The Kansas City English Bulldog Rescue (KCEBR) is a501 (c) 3 organization that rescues, rehabilitates and rehomes EnglishBulldogs as well as educates the public on this breed. Learn more about aboutKCEBR at: https://www.facebook.com/KCEBR

We'll have the Kentucky Derby on the big screen TV in the Auditorium.Andyou can't have a Kentucky Derby party without the traditional libation --we'll have Mint Juleps available! Kentucky Derby attire and hats optional butwelcome. We're looking forward to it!

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Saturday, May 6, 2017 - 7:30pm to Sunday, May 7, 2017 - 9:00pm
Weston Wine Company

In this best-selling book, author Deirdre Heekin tells herevolutionary story of turning her farm from overgrown fields to a fertile,productive, and beautiful landscape that melds with its natural environment.At this event, you’ll learn about all it takes to successfully grow winegrapes, and each attendee will leave with their very own vine so they can putwhat they learned to practice! Cost: $19/person or includedfor Wine Clubmembers. Wine Club members may RSVP for one of the two nights by calling usat 816.386.2345 or by sending an e-mail to [email protected];non-members reserve your spot at http://westonwinecompany.com/upcoming-events-2/

All supplies included, step by step instructions, glass of wine, and light snacks.

Tipple Hill Winery Vineyard- Color
Friday, March 25, 2016 - 6:30pm to 8:00pm
Tipple Hill Winery Vineyard

$10.00 per person- pre pay. Includes all supplies and a glass of wine.

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Saturday, March 26, 2016 - 7:00pm to 9:00pm
Tipple Hill Winery Vineyard

Music by Under the Big Oak Tree

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Sunday, March 27, 2016 - 1:00pm to 5:00pm
Tipple Hill Winery Vineyard

Easter picnic basket for two. $40.00

Includes- Basket, Fried Chicken, potato salad, fresh veggies, bread, a glass of wine each and a chocolate Easter Bunny.

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Friday, April 1, 2016 - 6:00pm to 8:00pm
Top Hat Winery

Larry is new to the Top Hat stage... Larry plays easy-going folk, country, Americana music. His songs consist of originals about family, friends, faith, regrets, winners, and losers. We are excited to have him playing so come out and support local!

Event URL: https://www.facebook.com/events/1541543522812561

Springfling
Saturday, April 2, 2016 - 11:00am to 5:00pm
Stone Hill Winery

A perfect combination of wine, shopping, and fun! Bringyour friends to sip and shop the day away!

April Fools Blind Tasting
Saturday, April 2, 2016 - 11:00am to Sunday, April 3, 2016 - 5:00pm
Jowler Creek Winery

So, you think you’re a wine aficionado and know your wines? Come put your superior tasting skills to the test and do a blind tasting of three red wines. We'll have a prize for those who guess correctly and wine specials even if we fool you! For the full prize information visit our website: http://www.jowlercreek.com/events.html . There’s no cost to play – invite your friends and come out and play Saturday or Sunday!

Paydirt Live at Blumenhof Winery
Saturday, April 2, 2016 - 2:00pm to 5:00pm
Blumenhof Winery

Paydirt (Rock Duo) will perform at Blumenhof Winery in Dutzow, MO on Saturday, April 2from 2-5pm.

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Somerset
9 December 2014

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An appeal to raise money for a baby girl who has lost all her limbs due to meningitis has reached £100,000.

Harmonie-Rose Allen from Foxhill, Bath, has been in Bristol Royal Children's Hospital since contracting meningococcal septicaemia in September.

She underwent life-saving treatment and will have to have more surgery after Christmas, doctors have said.

The money will be used for her future rehabilitation including hydrotherapy, physiotherapy and prosthetic limbs.

Doctors gave Harmonie-Rose a 10% chance of survival.

Her mother Freya Hall, 20, said the one-year-old's recovery and adaptation to life without limbs was remarkable.

"She's still my little bright child - my little clever girl," said Miss Hall.

Harmonie-Rose's father Ross Allen said they took her to the Bath Royal United Hospital after she woke up struggling to breathe.

Doctors could not find anything wrong and she was sent home.

She was taken back the next morning after having convulsions but again was sent home.

Hours later she was taken back after becoming lethargic and this time a rash like a bruise was found on her nose.

She was taken to Bristol Royal Hospital for Children by which time her legs and arms had turned black.

"It was the scariest moment of my life," said Mr Allen.

"I really didn't think she would survive the first night."

After spending a month in intensive care Harmonie-Rose began to get better.

"As soon as she woke up she decided to be here and to fight through this horrible disease," said Mr Allen.

She has since undergone more than 10 operations and faces further surgery until she is about 20, doctors have said.

She is using attachments on her stumps to play and is trying to feed herself.

"She knows something's happened and got frustrated at first but now she just wants to do things," said Miss Hall.

Mr Allen and Miss Hall said they were "overwhelmed" by the support they have received.

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More on this story

Video Meningitis baby Harmonie-Rose Allen to lose four limbs
21 October 2014
Video Fundraiser for meningitis baby Harmonie-Rose Allen
2 November 2014

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Bristol Royal Hospital for Children
Hope 4 Harmonie-Rose

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Figure 1. Comparison of cfDNA recovery using different commercially available kits.

A. Average cfDNA yield per 1 ml of plasma with 4 extraction kits, quantified using qPCR for GAPDH , ACTBL2 and HPRT and Qubit® 2.0 High Sensitivity dsDNA Assay. Error bar = ±SEM, n = 9 from 3 independent experiments each performed in triplicate. ND = Not detectable. B. Percent recovery of serial dilutions of λ/ Hind III DNA, quantified using assays targeting the 23 kb and 564 bp fragments. Error bar = ±SEM, n = 9 from 3 independent experiments performed in triplicate. CNA = Circulating Nucleic Acid kit.

https://doi.org/10.1371/journal.pone.0077963.g001

To determine the efficiency of cfDNA recovery, we assessed recovery of spiked-in λ/ Hind III DNA using the 3 kits that gave the highest yields of cfDNA (QIAamp ® DNA Blood Mini Kit, QIAamp ® CNA Kit and NucleoSpin ® Plasma XS Kit). The QIAamp ® DNA Blood Mini kit and the QIAamp ® CNA Kit showed similar but variable recovery of the 23kb fragment (range 20-70%). The QIAamp ® CNA Kit gave the highest yield of the smaller (564 bp) fragment, recovering between 90 - 100% at each dilution tested; whereas the NucleoSpin ® Plasma XS Kit showed a lower recovery of both amplicons ( Figure 1B ).

To determine whether the two kits giving the best recovery of cfDNA (QIAamp ® DNA Blood Mini kit and the QIAamp ® CNA Kit) showed any variance in cfDNA profiles, paired cfDNA from 4 metastatic patients were sequenced using the Ion AmpliSeq™Cancer Hotspot Panel v2 on the Ion PGM™ Sequencer. A total of 412M bases were generated across the eight cfDNA samples, with an average of 52 ± 5 M bases per sample ( Table S1 ). This was equivalent to 503 ± 46K reads, > 96% of which mapped to the target region providing an average coverage of 2000x. High similarity was observed in the coverage profile for each cfDNA pair across the 207 amplicons (average size 154 bp, range 111bp -187 bp) ( Hogan Interactive sneakers Blue Free Shipping Shop Latest Discount Professional Cheap Price Shop For For Sale Z5qtfpS0C
). Of note, one sample (P4) showed a peak in the number of reads (>30,000 reads) at ERBB2 suggesting gene amplification. The total number of reads at each amplicon showed high correlation, (ρ > 0.95, p <0.001) for 3 of the 4 cfDNA pairs ( Table S1 ). In the one sample (P3) with lower but significant correlation (ρ = 0.87) the QIAamp ® CNA method gave a more uniform coverage that the QIAamp ® DNA Blood Mini method.

Figure 2. Comparison of number of reads between two different extraction kits using the Ion AmpliSeq™Cancer Hotspot Panel v2.

Analysis of 4 plasma samples extracted using both the QIAamp ® DNA Blood Mini kit and the QIAamp ® CNA Kit. In each trace, the number of reads is shown on the Y-axis. Red lines indicate the QIAamp ® CNA Kit and the blue lines represent the QIAamp ® DNA Blood Mini kit. For in depth analysis see Tables S1 and S2 .

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